5.1 Preliminary check:
5.1.1 Check that the objectives, eyepieces and all external parts of the microscope are
clean.
5.2 Basic Operation:
5.2.1 Connect the instrument’s plug to the power supply and turn ‘ON’ the main switch
5.2.2 Switch ’ON’ the instrument switch, provided at the backside of the base.
5.2.3 Adjust the light brightness by rotating the knob.
5.2.4 Adjust the condenser by raising or lowering it.
5.2.5 Loosen the lock screw, turn the observation head to comfortable position for observation and then lock the lock-screw.
5.2.6 Place the slide on the mechanical stage.
5.2.7 Ensure that the specimen is centered over the stage, and adjust the specimen with coaxial knobs of the stage.
5.2.8 Turn the low power (10X) objective into position. Make sure it has clicked into position.
5.2.9 Lower the condenser as low as possible and open the iris diaphragm wide
5.2.10 Gradually raise the mechanical stage till the objective is almost touching the slide.
5.2.11 Then looking down the eye piece and using the coarse adjustment knob lower the mechanical stage so as to bring the object roughly into focus.
5.2.12 Use the fine adjustment knob to bring the object under microscope into sharp focus.
5.2.13 Make any final adjustment with the condenser and iris diaphragm if the high power objective is to be used, rotate the nosepiece until the high power objective occupies the position of the low power objective.
5.2.14 Next, use the coarse and fine adjustment knobs to focus on the object. Improve the light adjustment by raising the condenser and adjusting the diaphragm.
5.2.15 If a cover slip is necessary, it should be very thin or else it may not be possible to bring the objective close enough to focus the object.
5.3 Use of Oil Immersion Lens:
5.3.1 After focusing the object under low power, turn the nosepiece and put the oil immersion objective in place
5.3.2 Place a drop of oil (cedar wood) on the slide.
5.3.3 Gradually raise the mechanical stage till the oil drop touches the objective and just flattens it, without touching the object Itself.
5.3.4 During this procedure the objective should be viewed from the side with the eyes on level with the slide.
5.3.5 Now look down the ocular and slowly lower the mechanical stage by means of the coarse adjustment knob until the object is just in view Then using the fine adjustment knob brings the object into focus.
Note: Only 100 X objective is used as an oil immersion lens
5.4 Cleaning:
5.4.1 Clean the microspore with the help of the wet lint free cloth and dipped in cleaning agents.
5.4.2 Always Clean the microscope by switching OFF the mains power supply switch.