1.0 PURPOSE:
To define the procedure for cleaning of laboratory glassware used for analytical purpose, to avoid any cross contamination which results in erratic values.
2.0 SCOPE:
The procedure for cleaning of laboratory glassware is applicable to all glassware used in laboratory at (Company name).
3.0 REFERENCE:
4.0 DEFINITION:
5.0 RESPONSIBILITY:
5.1 Trainee/officer is responsible to perform activity.
5.2 Head-QC is responsible to ensure the compliance of the procedure.
6.0 PROCEDURE:
6.1 Daily Cleaning:
6.1.1 Empty the contents of glassware such as beakers, conical flasks, volumetric flasks, test tubes, measuring cylinders, pipettes, funnels, separating flasks, etc. carefully into the sink under the stream of water.
6.1.2 Rinse the glassware with tap water to remove any ruminants.
6.1.3 Dilute approximately 100 ml of 2.5 ml of Extran Neutral detergent solution with purified water. Pour this solution in the glassware and brush with smooth nylon brush.
6.1.4 Rinse with tap water 2 to 3 times till the soap ruminants are removed. For pipettes use hot water if required.
6.1.5 Keep the glassware for drying in hot air oven, after drying keep the them in designated place.
6.2 Monthly Washing Procedure:
6.2.1 Preparation of Nitric acid dilute: Contains approximately 10 per cent w/w of HNO3.
6.2.2 Dilute 106 ml of nitric acid to 1000 ml of water.
6.2.3 Clean all the glassware except calibrated/ graduated glassware with nitric acid once in a month or as and when required.
6.2.4 Pour small amount of nitric acid solution into the glass ware and rinse in such a way that all surface area get contacted with the acid solution and leave for an hour.
6.2.5 Subject the glassware to prolonged rinsing with tap water and finally rinse with purified water.
7.0 ABBREVIATIONS:
ml : milli liter.
HNO3. : Nitric acid.