1.0 PURPOSE:
To describe the procedure for cleaning of equipments and instruments in Microbiology Laboratory.
2.0 SCOPE:
This procedure is applicable to cleaning of equipments and instruments like Autoclave, incubators, hot air oven and refrigerator in the Quality Control (microbiology section), at (Company name).
3.0 DEFINITION:
4.0 RESPONSIBILITY:
4.1 Workmen is responsible for execution cleaning.
4.2 Trainee/officer-QC is responsible for checking of cleaning and procedure.
4.3 Head-QC is responsible for compliance of procedure.
5.0 FREQUENCY:
5.1 Autoclave: Outer cleaning with clean lint free cloth – daily.
Inner cleaning – after completion of last loaded cycle of the day.
5.2 Incubators and Hot air oven: Outer cleaning with clean lint free cloth – daily.
Inner sanitization with filtered 70% IPA – Weekly.
5.3 Refrigerator: Outer cleaning with clean lint free cloth – daily
Inner cleaning with purified water – Weekly
6.0 PROCEDURE:
6.1 Cleaning of autoclave:
6.1.1 Outer cleaning:
6.1.1.1 Everyday wipe the outer surface by using a clean lint free cloth.
6.1.2 Inner cleaning:
6.1.2.1 Ensure that the autoclave is switched off and the temperature is below 30°C.
6.1.2.2 Take a lint free cloth and dip it in cleaning agent (2.5% Extran), squeeze it and wipe the outer surface, inner surface and tray.
6.1.2.3 Dip the lint free cloth in purified water and wipe the area thoroughly.
6.1.2.4 Clean the drain point thoroughly with purified water and ensure that nothing is blocked in the point.
6.1.2.5 Then mop the inner surface with filtered 70% IPA solution thoroughly.
6.2 Cleaning of incubators and Hot air oven:
6.2.1 Outer cleaning:
6.2.1.1 Wipe the outer surface by using a clean lint free cloth daily.
6.2.2 Inner cleaning:
6.2.2.1 Shift the plates and tubes of a tray to the other tray.
6.2.2.2 Ensure that plates and tubes are not contaminated while transferring.
6.2.2.3 Dip the lint free cloth in purified water and wipe the door outer and inner surface, glass door outer and inner surface.
6.2.2.4 Then mop the inner surface area with filtered 70% IPA thoroughly.
6.2.2.5 Mop the trays with filtered 70% IPA and shift the plates to the cleaned tray.
6.2.2.6 Ensure that the incubating conditions shall not be affected during cleaning.
6.2.2.7 Take minimum time to clean the incubator, which shall not affect the incubating conditions.
6.3 Cleaning of Refrigerator:
6.3.1 Outer cleaning:
6.3.1.1 Wipe the outer surface by using a clean lint free cloth daily.
6.3.2 Inner cleaning:
6.3.2.1 Remove all the items from one shelf and transfer to the other shelf.
6.3.2.2 Ensure that the tubes, plates and flasks are not contaminated while transferring.
6.3.2.3 Dip the lint free cloth in purified water and wipe the area to remove the stains. Continue the process till the stains are completely removed from the surface.
6.3.2.4 Transfer all the items into cleaned shelf and clean the other shelf.
6.3.2.5 Clean the below tray, door inner surface and outer surface.
6.3.2.6 Take minimum time to clean the refrigerator, which shall not affect the cooling conditions.
7.0 ABBREVIATION:
IPA : Iso-Propyl Alcohol
% :Percentage